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KMID : 0602720040080030016
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Implantology
2004 Volume.8 No. 3 p.16 ~ p.27
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Effect of various dental materials on attachment of human osteoblast like cells in vitro
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Kim Chang-Su
Suh Kyu-Won
Shin Sang-Wan
Ryu Jae-Jun
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Abstract
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Statement of problem: Mechanisms of tissue-implant interaction and the effect of the implant surface on the behavior of cells has not yet been clarified.
Purpose: This study was performed to determine the effect of various dental materials on biologic responses of a human osteoblast-like cell line, MG63 in vitro.
Materials and methods: Osteosarcoma cell lines (MG63, Saos and G292) were cultured in the DMEM medium supplemented with fetal bovine serum. Among the three cells lines, MG63 proliferated the most rapidly. MG63 cells were cultured on various dental materials such as titanium, gold, alumina oxide single crystal, polystyrene, amalgam, composite resin and nickel. The morphology and attachment of the cells on the surface of gold and titanium of which were blasted with 50㎛ and 110㎛ alumina oxide were investigated. The attachment of cells were analyzed with a scanning electron microscope(SEM). The cell number on and around the material was counted with a hemocytometer.
Results: The appearances of the surfaces observed with SEM were different in the seven types of dental substrates. It was showed that MG63 cells were attached on titanium, gold, alumina oxide single crystal and polystyrene, but not on amalgam, composite resin and nickel. Unexpectedly, cells were hardly found on substrates blasted with alumina oxide.
Conclusion: It is concluded that the surface of dental materials can influence the attachment and growth of human osteoblast-like cells. It is likely that alumina oxide used to roughen the surface of the materials has a cytotoxic effect.
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KEYWORD
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MG63, SEM, Cell counting, Attachment
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